2 edition of Expression of proteinase inhibitor genes in transgenic plants found in the catalog.
Expression of proteinase inhibitor genes in transgenic plants
Javier Narvaez Vasquez
Written in English
|Statement||by Javier Narvaez Vasquez.|
|The Physical Object|
|Pagination||xiii, 104 leaves, bound :|
|Number of Pages||104|
Influence of wounding on the expression of the proteinase inhibitor II gene in leaves of transgenic tobacco plants. Tobacco plants (size: 80 cm) were wounded on one leaf by two consecutively. reported to mediate wound-induced proteinase inhibitor II (Pin2) gene expression in tomato and potato plants. Among changes in plant gene expression (19). Recently, strong evi- Transgenic potato plants containing a gene fusion consisting of a Pin2 promoterfused to a 3-glucuronidase.
A transgenic line (called J) containing a single T-DNA insertion was identiﬁed by Southern blot analysis. The binding proteins. A plasma membrane protein called phenotype of this line (constitutive accumulation of protein-SBP50 was shown to bind to the N-terminal part of ase inhibitor I, proteinase inhibitor II, and polyphenol oxi-systemin. "Expression of a chimeric proteinase inhibitor II-GUS gene in transgenic Solamim brevidens plants" is prepared to be submitted to the Journal of Plant Physiology, and the third paper, "Induction of cathepsin D inhibitor gene expression in response to methyl jasmonate" is submitted to Physiologia Plantarum.
Bowman-Birk inhibitor (BBI) genes encode serine protease inhibitors that have repetitive cysteine-rich domains with reactive sites for the trypsin or chymotrypsin family. We have identified seven BBI genes from japonica rice (Oryza sativa subsp. japonica var Teqing). All of the genes identified were found in a single cluster on the southern end of the long arm of rice . Both wounding of the plant as well as signaling molecules result in the formation of jasmonic acid, which then induces the gene expression of proteinase inhibitors. Many other signal cascades as well as the translocation of signal molecules through the phloem and xylem of the plant are also necessary for the production of these inhibitors.
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INTRODUCTION. Plant protease inhibitors (PIs) are small proteins, generally present at high concentration in storage tissues (up to 10% of protein content), but also detectable in leaves in response to the attack of insects and pathogenic microorganisms ().PIs’ contribution to plant defense mechanisms relies on inhibition of proteases present in insects’ guts or Cited by: Genes containing the cauliflower mosaic virus 35S promoter fused to open reading frames coding for tomato proteinase inhibitor I, tomato inhibitor II, and potato inhibitor II were expressed in transgenic tobacco plants.
Inhibitor I and II proteins were identified by immunoblotting and quantified by immunoradial by: Sanchez-Serrano JJ, Keil M, O'Connor A, Schell J, Willmitzer L. Wound expression of a potato proteinase inhibitor II gene in transgenic tobacco plants.
EMBO J. Feb; 6 (2)– [PMC free article] Schmidt WE, Ebel J. Specific binding of a fungal glucan phytoalexin elicitor to membrane fractions from soybean Glycine by: Following a brief survey of research on potato and tomato proteinase inhibitors and their role as defensive proteins, a study is described in which genes from potato and tomato encoding proteinase inhibitors I and II were incorporated into the genome of tobacco.
Leaves of transgenic tobacco plants containing high levels ( μg proteinase inhibitor protein/g Cited by: 4. Genes containing the cauliflower mosaic virus 35S promoter fused to open reading frames coding for tomato proteinase inhibitor I, tomato inhibitor II, and potato inhibitor II were expressed in transgenic tobacco plants.
Inhibitor I and II proteins were identified by immunoblotting and quantified by immunoradial diffusion. Both inhibitors exhibited the molecular weights found for Cited by: The introduction and expression of proteinase inhibitor encoding genes into sugarcane (Saccharum officinarum L.) genome is an interesting strategy for conferring partial resistance to the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae), the major insect pest of sugarcane in investigate the role of soybean (Glycine max L.) Kunitz trypsin inhibitor.
Title: Prospects for Using Proteinase Inhibitors to Protect Transgenic Plants Against Attack by Herbivorous Insects VOLUME: 12 ISSUE: 5 Author(s):John A. Gatehouse Affiliation:School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK.
Keywords:Bt/CpTI cotton, crop protection, genetic modification, plant-insect interactions, protein proteinase inhibitor. We introduced the potato proteinase inhibitor II (PINII) gene (pin2) into several Japonica rice varieties, and regenerated a large number of transgenic rice plants.
Wound-inducible expression of. McGregor P.G.'s 12 research works with citations and reads, including: Transgenic Pest and Disease Resistant White Clover Plants. In this study we examined the effect of the expression of two proteinase inhibitors, belonging to different families, against T.
absoluta and its impact on the endogenous defensive response in tomato plants. We report that the co-expression of both proteinase inhibitors had an additive effect and enhanced tomato plants resistance against T.
The expression of seven novel candidate genes of Sugar transporter (XLOC_), Major intrinsic protein (XLOC_), Proteinase inhibitor I25, cystatin, conserved region (XLOC_), Plant peroxidases (XLIC_ and XLOC_), Ethylene responsive transcription factors (XLOC_ and XLOC_) in infected.
Protease Inhibitors HIV Protease Inhibitors Serine Proteinase Inhibitors HIV Protease Serine Endopeptidases Peptide Hydrolases Secretory Leukocyte Peptidase Inhibitor Endopeptidases Saquinavir Indinavir Ritonavir Proteinase Inhibitory Proteins, Secretory Trypsin Inhibitors Nelfinavir Serpins Cysteine Proteases Cysteine Endopeptidases Cysteine.
Results. The CPI gene, designated JcCPI, was cloned from ; its sequence shared conserved domains with other plant on a transcription pattern analysis, JcCPI was expressed in all tissues examined, but its expression was highest in the petiole.
Additionally, the expression of JcCPI was induced by salinity stress. A potential role of JcCPI was detected in transgenic E. JASMONATE SIGNALING FOR PROTEINASE INHIBITOR GENES plants (Fig. Methyl jasmonate induced the synthesis of inhibitorI andII proteinsandmRNAin amannersimilarto wounding, which is knownto transcriptionally regulate syn-thesis of the inhibitor mRNAs(5).
In similar experiments with alfalfa plants exposed to methyljasmonate vapor, the. We found that larvae fed on tomato transgenic plants co-expressing both proteinase inhibitors showed a notable reduction in weight.
Moreover, only 56% of these larvae reached the adult stage. The emerged adults showed wings deformities and reduced fertility. In many plants, cysteine proteinases play essential roles in a variety of developmental and physiological processes. In rice (Oryza sativa), REP-1 is a primary cysteine proteinase responsible for the digestion of seed storage proteins to provide nutrients to support the growth of young seedlings.
In the present study, the gene encoding REP-1 was isolated, characterized. Whereas no or little expression of the proteinase inhibitor II gene could be detected in non-wounded leaves, high levels of proteinase inhibitor II mRNA were detected in leaves of several transgenic tobacco plants after mechanical wounding as well as after treatment of detached leaves with oligosaccharides.
The protease inhibitors (PIs) in plants are involved primarily in defense against pathogens and pests and in response to abiotic stresses. However, information about the PI gene families in tomato (Solanum lycopersicum), one of the most important model plant for crop species, is limited.
In this study, in silico analysis identified 55 PI genes and their conserved domains. The effect of progressive 5[prime] deletions within a potato proteinase inhibitor II promoter on wound-inducible expression of the chloramphenicol acetyltransferase (CAT) gene in leaves of transgenic tobacco plants was analyzed.
After deletion of a region ranging from position to with respect to the transcription start site, promoter activity was markedly reduced but still.
Abstract. A chimeric gene consisting of kb of the 5' regulatory region of a member of the potato proteinase inhibitor II gene family, the coding region of the bacterial β-glucuronidase (GUS) gene and bp of the proteinase inhibitor II 3'-untranslated region containing the poly(A) addition site was introduced into potato and tobacco by Agrobacterium tumefaciens.
CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The effect of progressive 5 ‘ deletions within a potato proteinase inhibitor II promoter on wound-inducible expression of the chloramphenicol acetyltransferase (CAT) gene in leaves of transgenic tobacco plants was analyzed.
After deletion of a region ranging from position to with .ADVERTISEMENTS: The below mentioned article provides a study note on transgenic plants.
The application of recombinant technique for horizontal transfer of gene in plant system has several limitations. Plant genome is exceedingly complex, involving a huge amount of DNA, which can accommodate sequences equivalent to million genes in an average crop plant.
Several genes .As the processing mechanism of all known potyviruses involves the activity of cysteine proteinases, we asked whether constitutive expression of a rice cysteine proteinase inhibitor gene .